Future University In Egypt (FUE)
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Altagamoa Al Khames, Main centre of town, end of 90th Street
New Cairo
Egypt

AMANY AHMED RABEE MOHAMED OSMAN

Basic information

Name : AMANY AHMED RABEE MOHAMED OSMAN
Title: Professor
Google Schoolar Link
Personal Info: Amany Ahmed Rabea works as a Associate Professors at the Faculty of Dentistry at Future University in Egypt

Education

Certificate Major University Year
PhD Oral Biology 2012
Masters Oral Biology 2009
Bachelor 2002

Researches /Publications

Ultra-Performance Liquid Chromatography Coupled with Mass Metabolic Profiling of Ammi majus Roots as Waste Product with Isolation and Assessment of Oral Mucosal Toxicity of Its Psoralen Component Xanthotoxin

AMANY AHMED RABEE MOHAMED OSMAN

Alshaimaa M. Almehmady ; Hanaa Alkharobi ; Sameh S. Elhady

29/09/2023

https://www.mdpi.com/2218-1989/13/10/1044

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An Overview About Mesenchymal Stem Cell Exosomes and their Applications in Biological Research

AMANY AHMED RABEE MOHAMED OSMAN

24/07/2023

https://ejh.journals.ekb.eg/article_309475.html

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Therapeutic Effect of Curcumin, Ginger and Tamarind on Oral and Paraoral tissues: Histological Overview

AMANY AHMED RABEE MOHAMED OSMAN

06/06/2023

https://digitalcommons.aaru.edu.jo/cgi/viewcontent.cgi?article=1209&context=fdj

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Regenerative capacity of bone marrow stem cells on aged albino rat’s parotid excretory duct

AMANY AHMED RABEE MOHAMED OSMAN

Laila Rashed; Rabab Hassan

01/09/2022

https://www.sciencedirect.com/science/article/abs/pii/S0003996922001273

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The prospective Effect of Cinnamon and Chia on Submandibular Salivary Glands after Ciprofloxacin Administration in Albino Rats

AMANY AHMED RABEE MOHAMED OSMAN

Medhat A. Elzainy; iman A. Fathy

05/07/2022

https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/prospective-effect-of-cinnamon-and-chia-on-submandibular-salivary-glands-after-ciprofloxacin-administration-in-albino-rats-histological-histochemical-and-ultrastructural-study/905059489CDD847CABE7D7DEB4D99B0E

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Assessment of ultra-structure, viability and function of lipopolysaccharides-stimulated human dermal fibroblasts treated with chrysin and exosomes (in vitro study)

AMANY AHMED RABEE MOHAMED OSMAN

Rabab Hassan; Dina Sabry

01/04/2022

https://www.sciencedirect.com/science/article/pii/S1013905222000530

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Histological, Fluorescence and Ultrastructural Assessment of Presumptive Effect of Carbimazole Treatment and its Co-administration with Bone Marrow-Derived Mesenchymal Stem Cells on Parotid Glands of Albino Rats

AMANY AHMED RABEE MOHAMED OSMAN

Heba A. Adawy

01/05/2021

Background: Carbimazole is a popular drug for hyperthyroidism. However, it has many side effects on different tissues. Bone marrow-derived mes‌enchymal stem cells (BM-MSCs) are efficient in tissue regeneration. Aim: To appraise the consequence of carbimazole (antithyroid drug) intake on albino rat parotid gland structure and plausible outcome of bone marrow-derived mes‌enchymal stem cells (BM-MSCs) application at different durations. Material and methods: Forty adult male albino rats were used and categorized into: Group I: obtained distilled water. Group II: acquired therapeutic dose of carbimazole. Group III: received carbimazole and single dose of BM-MSCs at the start of experiment. Group IV: got carbimazole then by finishing of 3rd week they received solitary injection of BM-MSCs. Preparation of specimens for examination by light, fluorescent and transmission electron microscope was performed. Histomorphometric data of acini area% was statistically analyzed using ANOVA test. Results: Both histological and ultrastructural examinations illustrated that parotid gland has normal structure in Group I and approximately normal features in Group III. Group II demonstrated distorted acini and duct system. Group IV presented normal features in some acini and some areas of duct system but degenerative features in others. Results of fluorescence labeling explored some labeled-BM-MSCs in Group III but apparently copious labeled cells in Group IV. Statistical results showed highest mean acini area% in Group I, subsequently Group III, then Group IV, followed by Group II. Conclusions: Carbimazole has deteriorative outcomes on the parotid gland of albino rats. BM-MSCs ameliorate the damaging upshot of carbimazole in a direct proportion manner with time factor.

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The prospective role of mesenchymal stem cells exosomes on circumvallate taste buds in induced Alzheimer's disease of ovariectomized albino rats: (Light and transmission electron microscopic study)

AMANY AHMED RABEE MOHAMED OSMAN

RababHassan, Dina Sabry

01/10/2019

OBJECTIVE: To elucidate the effect of Alzheimer's disease on the structure of circumvallate papilla taste buds and the possible role of exosomes on the taste buds in Alzheimer's disease. DESIGN: Forty two ovariectomized female adult albino rats were utilized and divided into: Group I: received vehicle. Group II: received aluminum chloride to induce Alzheimer's disease. Group III: after the induction of Alzheimer's disease, each rat received single dose of exosomes then left for 4 weeks. The circumvallate papillae were prepared for examination by light and transmission electron microscope. STATISTICAL ANALYSIS: histomorphometric data were statistically analyzed. RESULTS: Histological examination of circumvallate papilla in Group I showed normal histological features. Group II revealed distorted features. Group III illustrated nearly normal histological features of circumvallate. Silver impregnation results showed apparently great number of heavily impregnated glossopharyngeal nerve fibers in both Groups I & III but markedly decreased in Group II. Synaptophysin-immunoreactivity was strong in Group I, mild in Group II and moderate in Group III. The ultra-structural examination of taste bud cells revealed normal features in Group I, distorted features in Group II and almost normal features in Group III. Statistically highest mean of Synaptophysin-immunoreactivity area% was for Group I, followed by Group III, and the least value was for Group II. CONCLUSIONS: Alzheimer's disease has degenerative effects. Bone marrow mesenchymal stem cell (BM-MSC)-derived exosomes have the ability to improve the destructive changes induced by Alzheimer's disease.

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Gingival pigmentation (cause, treatment and histological preview)

AMANY AHMED RABEE MOHAMED OSMAN

01/06/2017

Facial appearance depends on several oral and extraoral factors. The gingiva is an important intraoral tissue which when affected particularly by pigmentation is mainly responsible for the unpleasant appearance. Several causes of gingival pigmentation were previously mentioned in text together with the possible techniques of treatment. In this review, we will focus on this topic with a histological point of view.

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COMPARATIVE STUDY ON THE POSSIBLE EFFECT OF COD LIVER OIL VERSUS INSULIN ON PAROTID SALIVARY GLANDS OF STREPTOZOTOCIN-INDUCED DIABETIC ALBINO RATS

AMANY AHMED RABEE MOHAMED OSMAN

01/01/2017

Background: The diabetic condition is frequently associated with impaired functions of salivary glands. testified by both morphological deterioration of the gland and by altered salivary composition. Abnormal apoptosis has been implicated in salivary glands of diabetic rat models. Amyloidosis constitutes a group of diseases in which proteins deposit in tissues as insoluble fibrils, causing progressive organ dysfunction. Although insulin and oral hypoglycemic agents are the mainstays of diabetes treatment, they have prominent side effects and fail to alter the course of diabetic complications. Cod liver oil (CLO) is an important source of long-chain omega-3 (co-3) fatty acids as well as vitamins A. E and D. CLO has antioxidant effect especially on parotid salivary glands. Objective: The present study was undertaken to investigate the possible role of CLO versus insulin supplementation in enhancement of parotid salivary glands in streptozotocin (STZ)-induced diabetic rats. Design: Sixty adult male Swiss albino rats (200-250 gm) were selected for this study. The ani-mals were randomly divided into four groups (fifteen rats each): Group I (Control group). Group II (Diabetic untreated group). Group DI (Insulin treated group) and Group IV (Cod liver oil treated group). At the end of the experimental period (four weeks). the rats were sacrificed and the parotid salivary glands were dissected out. The sections were examined histologically. immunohis-tochemically. histozoorpbometrically and by fluorescence staining technique. Statistical analysis: Data obtained from histomorphomettic analysis were statistically described in terms of mean standard detnion (x SD). Results: Histopathologic examination of Group I showed the normal histological features of parotid gland. Group II revealed apparent reduction in acinar size, ill-defined acinar and ductal cells outlines, nuclear changes. acinar and ductal cells degeneration, lipid droplets. dilatation of the duct system lumina and stagnated salivary secretion in the lumina of striated and excretory ducts. Moreover apparent decrease, hyalinization and degeneration in the fibrous connective tissue (Cl)

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THE POSSIBLE EFFECT OF OMEGA-3 FATTY ACIDS VERSUS INSULIN ON EXPRESSION OF ACTIVATED CASPASE-3 AND RUSSELL BODIES DEVELOPMENT IN BUCCAL MUCOSA OF STREPTOZOTOCIN-INDUCED DIABETIC ALBINO RATS

AMANY AHMED RABEE MOHAMED OSMAN

Marwa M.Abdel-Hameed

01/01/2017

Background: Damage of tissue repair and dysfunction of the oral mucosa are usually associated with the diabetic conlition.Apoptosis plays a functional role in limiting diabetic repair. Russell bodies, an uncommon finding that may be associated with chronic inflammatcry conditions. Although insulin is the mainstay of diabetes treatment, it has prominent side effects. Episodes of severe hypoglycemia and increased mortality rate are associated with insulin therapy. Cod liver oil (CLO) is an important source of long-chain omega-3 (o-3) fatty acids that have antioxidant and anti-inflammatcry properties. Objective: The present study was designed to determine the adaptive apoptotic alterations accompanying diabetes in the buccal mucosa with the possibility of occurrence of the so-called Russell bodies and the passible role of long-chain ro-3 fatty acids versus insulin supplementation in enhancement of the buccal mucosa in streptonaccin (STZ)-induced diabetic rats. Design: Sixty adult male Swiss albino rats (X00-250 gm) were selected for this study. The animals were randomly divided into four groups (fifteen rats each): Group I (Control group), Group II (Diabetic untreated snip), Group Ill (Insulin treated group) and Group IV (Cod liver oil treated group). At the end of the experimental period (four weeks), the rats were sacrificed and the specimens were obtained from the mucosa of the cheek of both sides. The sections were examined histologically, immunohistochemically and histomorphometrically. Statistical analysis: Data obtained from histomorphometric analysis were statistically described in teams of mean standard cleaiation (2 SD). Results: Histopathologic examination of Group I revealed the no histological features of the buccal mucosa. In Group II several histological changes in the epithelial layer of the buccal mucosa were noticed. These changes include; atrophy in the epithelium, evidences of cells undergo degeneration, nuclear changes, ill-defined cell membrane, croplasonc' exudations,

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Comparative Study of the Possible Effect of Cow Milk and Soy Milk on Enamel of Human Premolar Samples

AMANY AHMED RABEE MOHAMED OSMAN

Lobna M. Nabil, Medhat A. El-Zainy

01/07/2016

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Variations in Cemento-Enamel Junction on Different Aspects in a Sample of Egyptian Premolars

AMANY AHMED RABEE MOHAMED OSMAN

Dina M. Abdel-Khalik

01/07/2016

Several studies reviewed the morphology of cemento-enamel junction (CET) in permanent den-tition. These studies have questioned the validity of the data found in twLibooks of oral histology and have hiebliebted deficiencies in ow current understanding of the morphology and distribution of the mineralized tissues that compose the CEJ. The location and nature of the CV are more than descriptive terms used simple to descnbe some aspects of tooth morphology since they have defi-nite clinical significance. Aim: The aim of the present study was to determine the interrelationship of the mineralized tissues that compose the CET in the various surfaces of the Egyptian premolars as well as the chemical profile in each type. Materials and methods: The CEJ in a sample of 50 caries and defect-free human premolars enacted for orthodontic reasons was examined using transmitted light microscope and environmental scanning electron microscope (SEM). The different CET interrelationships were chemically analyzed to determine surface calcium (Ca). phosphorus (P) and carbon (C) wt% using energy-dispersive X-ray analyzer (EDXA). The EDXA chemical profile rest and distribution of different CHI interrelationships at different surfaces were statistically analyzed.

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VARIATION IN EROSION SUSCEPTIBILITY BETWEEN BUCCAL AND MESIAL SURFACES OF HUMAN PREMOLAR ENAMEL

AMANY AHMED RABEE MOHAMED OSMAN

Ahmed M. Halawa

01/07/2015

The increased consumption of carbonated soft drinks is becoming more important because of the concern for dental erosion. Erosive enamel lesions most commonly affect the cervical part of facial surfaces of teeth. The largest segment of the soft drink industry is the colas. Aim: The present research aimed to compare the erosive effect of Coca-Cola® beverage between the buccal and mesial cervical enamel of human premolars. Material and methods: Twenty extracted sound human maxillary first premolars were used in the present study. Ten premolars served as Reference group (group I): Teeth of this group were utilized to provide ground sections of normal enamel to serve as reference for the control group (Group II). The other ten premolars were utilized as Control group (group II): Teeth of this group were examined and chemically analyzed to determine surface calcium (Ca), phosphorus (P) and carbon (C) weight percentage (wt%) using environmental scanning electron microscope attached with energy dispersive x-ray analyzer (SEM-EDXA). Coca- Cola® experimental group (group III): in which the teeth of (Group II) were immersed in Coca-Cola® and re-examined using SEM-EDXA then ground sectioned for light microscopic examination. Morphometric study: was performed for assessment of the surface affection band thickness. Statistical analysis: was performed to assess the effect of surface difference and application of Cola® on Ca, P and C wt%. Results: The light microscopic results revealed that the control mesial cervical enamel was relatively thinner and more rapidly tapering cervically than buccal cervical enamel. In subsurface enamel, the mesial side presented varying diameter enamel rods with apparently thicker inter-rod regions. After exposure to Cola®, both buccal and mesial cervical thirds of group (III) showed an apparent decrease in the enamel thickness with apperance of thick dark band at enamel surface. In the subsurface enamel, both enamel rods and the interrod regions appeared more accentuated. These changes were more obvious mesially than buccaly. Morphometric results: for group III revealed that the mesial cervical enamel showed greater mean measures of the surface affection band than the buccal one. SEM examination of enamel in the buccal cervical third of group (II), revealed plenty of perikymata grooves presenting shallow enamel rod ends & ridges of rodless enamel. Mesial cervical third of group (II), revealed more frequent perikymata grooves with more numerous rod ends & ridges, with narrower areas of rodless enamel. Scanning electron micrographs of enamel in the buccal cervical third of group (III), showed an evident generalized poorly defined surface structure. The mesial cervical enamel of group (III) showed noticeably irregular surface with variable sized small depressions. Statistical analysis: for EDXA data of groups II and III revealed statistically significant higher mean Ca and P wt % and a lower C wt % in the buccal cervical third than those of the mesial one. Moreover, both buccal and mesial cervical parts of group III showed significantly reduced mean Ca and P wt % and elevated C wt % than their correspondents of group II. Comparing the Cola® induced changes in wt% of the studied elements between buccal and mesial surfaces showed that the changes were significantly higher in the mesial one. Conclusions: 1- Mesial cervical enamel normally differs from its buccal counterpart in terms of surface and subsurface structure as well as its elemental composition. 2- Cola® beverage obviously affected the tested parameters in both buccal as well as mesial cervical thirds. 3- The mesial cervical enamel presented greater affection than its buccal counterpart.

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PREVALENCE OF THE TWO VARIANTS OF MANDIBYLAR SECOND PREMOLARS IN THE EGYPTIAN POPULATION

AMANY AHMED RABEE MOHAMED OSMAN

Reham M. Amin

01/01/2015

Three hundred and four Egyptian cases (608 mandibular second premolars) were screened from the out -patient clinic of Future University in Egypt Dental Hospital. Morphological features of the crown, number and position of cusps as well as the sex of the patient were recorded clinically. The females showed statistically significant higher prevalence of bilateral lower second premolar three cusp type (3 C.T) than males and the vice versa was seen for the males. On the other hand there was no sex predilection in the prevalence of the mixed forms. In both sexes the bilateral 2 C.T variant of lower second premolar was statistically significant higher than the bilateral 3 C.T. However the bilateral variants are statistically significant higher than the mixed ones. In both sexes the 3 C.T variant of lower second premolar in the right side was statistically significant higher than that of the left side.

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Comparative study of onion versus Miacalcic on tooth supperting tissues of albino rats treated by Dexamethasone

AMANY AHMED RABEE MOHAMED OSMAN

01/07/2014

Objective: The purpose of this study is to compare the effect of onion and miacalcic on alveolar bone, periodontal ligament (PDL) and cellular cementum in albino rats treated with dexamethasone. Design: Forty male Swiss albino rats ranging from 150-200 gm were selected for the study and randomly divided into four groups: Group I (Control group), Group II (Dexamethasone treated group), Group III (Dexamethasone and Miacalcic treated group) and Group IV (Dexamethasone and Onion treated group). At the end of the experimental period (four weeks), the rats were sacrificed and the mandible specimens were divided into right and left halves. Half of the samples were kept for measuring bone mineral density (BMD). The other halves were prepared for light microscopic examination and immunohistochemical assessment. Results: Histopathological examination of group II revealed severe bone and cementum resorption and disorganization of PDL. Less resorptive activity and more dense and organized PDL could be detected in group III, while group IV showed normal architecture closely resembling that of the control group. The greatest fibronectin (FN) expression and the highest mean of BMD values were demonstrated in the onion treated group (group IV) which was nearly similar to that of the control group. Conclusions: Onion treatment when combined with dexamethasone prevented alveolar bone and cementum resorption to a marked degree, preserved the density of the alveolar bone, and maintained the PDL architecture to a level comparable to the control.

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The effect of some carbonated beverages on enamel of human premolars (Scanning and Light Microscopic Study).

AMANY AHMED RABEE MOHAMED OSMAN

01/01/2012

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Histochemical and Histological study on the possible radioprotective effect of acetyl salicylic acid (Aspirin) on the percentage of survival cells in the parotid and submandibular salivary glands of irradiated rats.

AMANY AHMED RABEE MOHAMED OSMAN

Medhat A. El-Zainy, Reham M. Amin

01/01/2009

The aim of thiswork is to study the possible radioprotective effect of acetyl salicylic acid (Aspirin) on the percentage of survival cells in the parotid and submandibular salivary glands of irradiated rats.

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Awards

Award Donor Date
Future University Award for Scientific Research Future University 2013

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