Mona Fathy Abdel Maksoud Eldeeb

Alshaimaa M. Almehmady ; Hanaa Alkharobi ; Sameh S. Elhady

29/09/2023

https://www.mdpi.com/2218-1989/13/10/1044

Mona Fathy Abdel Maksoud Eldeeb

24/07/2023

https://ejh.journals.ekb.eg/article_309475.html

Mona Fathy Abdel Maksoud Eldeeb

06/06/2023

https://digitalcommons.aaru.edu.jo/cgi/viewcontent.cgi?article=1209&context=fdj

Mona Fathy Abdel Maksoud Eldeeb

Maha El Shahawy

01/07/2022

https://www.sciencedirect.com/science/article/pii/S1013905222000566

Mona Fathy Abdel Maksoud Eldeeb

Dina Maged Eldahrawy, Heba A. Adawy

01/03/2021

Background: Hypothyroidism is a clinical disorder that results from insufficient production of thyroid hormones leading to a total decrease of metabolic demands of the body and causing serious abnormalities. Hypothyroidism is known to affect salivary glands causing deleterious changes including apoptosis and numerous cytoplasmic vacuolation. Mesenchymal stem cells (MSCs) have high capability of self-renewal. Thus, have enormous therapeutic potential for tissue repair. Objective: The study aimed to assess the influence of bone marrow derived mesenchymal stem cells (BM-MSCs) on the histological ultrastructure of submandibular salivary gland of carbimazole-induced hypothyroidism in rats. Method: Twenty one adult male albino rats were divided into three groups (seven rats each). Group I: Is considered as control group. Group II: (Carbimazole induced hypothyroidism group): animals were given a single daily oral dose of carbimazole (5mg/250g/day) dissolved in 3 ml of distilled water delivered by intragastric tube for five weeks, to induce hypothyroidism. Group III: (BM-MSCs treated group): hypothyroidism was induced similar to group II. Then rats were injected at the lateral tail vein with 1x 107 BM-MSCs cells in 0.2ml phosphate buffer saline immediately following induction of hypothyroidism. All rats were euthanized after 8 weeks (5 weeks of hypothyroidism induction + 3 weeks of stem cells injection). Submandibular salivary glands were prepared for transmission electron microscopic examination (TEM). Results: Ultrastructural results of group II revealed massive degenerative changes within the acinar and ductal cells’ nuclei and organelles, together with disruption of their intercellular junctions. Marked improvement of parenchymal cells’ architecture was detected with BM-MSCs administration in group III. Conclusion: BM-MSCs have the potentiality to repair salivary glands damage following induction of hypothyroidism. Background: Hypothyroidism is a clinical disorder that results from insufficient production of thyroid hormones leading to a total decrease of metabolic demands of the body and causing serious abnormalities. Hypothyroidism is known to affect salivary glands causing deleterious changes including apoptosis and numerous cytoplasmic vacuolation. Mesenchymal stem cells (MSCs) have high capability of self-renewal. Thus, have enormous therapeutic potential for tissue repair. Objective: The study aimed to assess the influence of bone marrow derived mesenchymal stem cells (BM-MSCs) on the histological ultrastructure of submandibular salivary gland of carbimazole-induced hypothyroidism in rats. Method: Twenty one adult male albino rats were divided into three groups (seven rats each). Group I: Is considered as control group. Group II: (Carbimazole induced hypothyroidism group): animals were given a single daily oral dose of carbimazole (5mg/250g/day) dissolved in 3 ml of distilled water delivered by intragastric tube for five weeks, to induce hypothyroidism. Group III: (BM-MSCs treated group): hypothyroidism was induced similar to group II. Then rats were injected at the lateral tail vein with 1x 107 BM-MSCs cells in 0.2ml phosphate buffer saline immediately following induction of hypothyroidism. All rats were euthanized after 8 weeks (5 weeks of hypothyroidism induction + 3 weeks of stem cells injection). Submandibular salivary glands were prepared for transmission electron microscopic examination (TEM). Results: Ultrastructural results of group II revealed massive degenerative changes within the acinar and ductal cells’ nuclei and organelles, together with disruption of their intercellular junctions. Marked improvement of parenchymal cells’ architecture was detected with BM-MSCs administration in group III. Conclusion: BM-MSCs have the potentiality to repair salivary glands damage following induction of hypothyroidism.

Mona Fathy Abdel Maksoud Eldeeb

Heba Mohammad Hakam, Rehab Ali Abdel Moneim

01/09/2020

Background: Bone defects resulting from trauma, tumor resection, infection, and congenital or acquired deformities remains an important clinical problem. Synthetic nano-crystalline hydroxyapatite, Nano bone, was successfully used in healing of bone defects without revealing negative side effects. Biodentine; a calcium-silicate based material was reported to have osteogenic and angiogenic properties. Objectives: This study aims to investigate the initial osteoinductive potential of Nano Bone and Biodentine on surgically created defects in rabbit’s alveolar process. Methods: 30 adult male rabbits (1-1.5kg) were used in this study. Bilateral bone defects were created in the mandibles of all rabbits, one in each side; the right sides were experimental, and the lefts were kept empty as control. Animals were then divided into two groups (15 rabbits each); Group I (Biodentine): The right-side defects were loaded with Biodentine material. Group II (Nano Bone): Nano Bone was packed in the right-side defects. Five rabbits were euthanized from each group at; 3, 7 and 14 days postoperatively. Bone defects’ specimens were prepared for histological examination by light microscope as well as quantitative analysis of gene expression of collagen1 alpha and Runx-2 by real time PCR. Results: Biodentine had initiated osteogenesis; yet the newly formed bone was apparently of lesser quality than that formed with Nano Bone. Runx- 2 showed significant increase in Nano Bone compared to Biodentine at 1 week, while collagen1 alpha gene expression was significantly increased at all intervals. Conclusion: Both Nano Bone and Biodentine had initiated osteogenesis. Nano bone showed better healing results when compared to Biodentine.

Mona Fathy Abdel Maksoud Eldeeb

Fatma Adel Saad; Rehab Abdel Moneim

01/03/2020

Background: A correlative characterization of oral mucosa was carried out in common animal species. Objective: This work aimed to correlate the histological structure of lingual gustatory papillae as well as the immunohistochemical reactivity to a particular umami receptor among different animal species to categorize the best experimental animal models for research. Methods: The dorsal lingual mucosal specimens were obtained from four species (orders) including; chicken (Galliformes), frogs (Anura), camels (Artiodactyla) and rabbits (Lagomorpha). They were processed for routine histological examination; histochemical staining using periodic acid Schiff (PAS) and Masson’s trichrome in addition to immunohistochemical localization of umami metabotropic glutamate receptor-4 (mGluR4) antibody. Results: Chicken, camels and rabbits exhibited keratinized stratified epithelium on the dorsal lingual mucosa with statistically greatest thickness in anterior lingual epithelium of chicken. For frogs, filiform and fungiform papillary walls were formed of mucous secreting columnar monolayer epithelium with a subjacent spindle cell layer. Insignificant differences in PAS staining intensity of dorsal lingual epithelium were noted between chicken anteriorly and rabbits as well as between chicken posteriorly and camels with the greatest significant intensity in frogs reflecting the highest content of glycogen and mucin. Likewise, the density of lamina propria and degree of collagen fibers bundling detected by Masson's trichrome were significantly different among species greatest in chicken and least in frogs. Intraepithelial taste buds were found in chicken while frogs displayed on top of fungiform papillae the largest gustatory disc among vertebrates. Camels and rabbits presented conventional papillary taste buds with the absence of foliate papillae in camels. Chicken and camel were negatively immunoreacted to mGluR4; frogs and rabbits were positively immunoreacted with the strongest reaction in rabbits. Conclusion: It was concluded that the direct association between histological variations of masticatory lingual mucosa and diverse environmental factors would reflect the adaptation capability of the lingual tissue.

Mona Fathy Abdel Maksoud Eldeeb

01/10/2019

Background: A correlative characterization of oral mucosa was carried out in common animal species. Objective: This work aimed to correlate the histological structure of lingual gustatory papillae as well as the immunohistochemical reactivity to a particular umami receptor among different animal species to categorize the best experimental animal models for research. Methods: The dorsal lingual mucosal specimens were obtained from four species (orders) including; chicken (Galliformes), frogs (Anura), camels (Artiodactyla) and rabbits (Lagomorpha). They were processed for routine histological examination; histochemical staining using periodic acid Schiff (PAS) and Masson’s trichrome in addition to immunohistochemical localization of umami metabotropic glutamate receptor-4 (mGluR4) antibody. Results: Chicken, camels and rabbits exhibited keratinized stratified epithelium on the dorsal lingual mucosa with statistically greatest thickness in anterior lingual epithelium of chicken. For frogs, filiform and fungiform papillary walls were formed of mucous secreting columnar monolayer epithelium with a subjacent spindle cell layer. Insignificant differences in PAS staining intensity of dorsal lingual epithelium were noted between chicken anteriorly and rabbits as well as between chicken posteriorly and camels with the greatest significant intensity in frogs reflecting the highest content of glycogen and mucin. Likewise, the density of lamina propria and degree of collagen fibers bundling detected by Masson's trichrome were significantly different among species greatest in chicken and least in frogs. Intraepithelial taste buds were found in chicken while frogs displayed on top of fungiform papillae the largest gustatory disc among vertebrates. Camels and rabbits presented conventional papillary taste buds with the absence of foliate papillae in camels. Chicken and camel were negatively immunoreacted to mGluR4; frogs and rabbits were positively immunoreacted with the strongest reaction in rabbits. Conclusion: It was concluded that the direct association between histological variations of masticatory lingual mucosa and diverse environmental factors would reflect the adaptation capability of the lingual tissue.

Mona Fathy Abdel Maksoud Eldeeb

01/07/2018

Diabetic patients suffer from several oral complications: thus there has been unending efforts in searching for treatment agents either in synthetic forms or from plant sources. The aim of the present study is to prove the efficacy of aqueous tamarind seeds extract in treatment of the complications that might occur in tongue papillae of high fat diet/streptozotocin (HFISTZ) type 2 diabetic rats relative to the conventional anti-diabetic drugs. Design: Thirty five adult male albino rats (200.220 gm) were selected for this study. The animals were randomly divided into five groups (seven rats each). Group I (Control -ve). Group II (Diabetic control): Type 2 diabetes was induced by 58% calories HF diet for 4 weeks followed by intraperitoneal administration of 517 (35 mg/ kg). Two weeks after STZ injection. diabetic rats were treated with oral doses of antidiabetic drugs as follows: Group III: Metformin (500mg/I ml distilled water/ kg/twice daily). Group IV: Forxiga (0.1 mg/I ml distilled water/ kg/day) and Group V: Aqueous tamarind seeds extract (80 mc0.5 ml distilled water/I00 g/day) for 4 weeks. Blood glucose level was measured every week. At the end of the experiment, tongue specimens were dissected and processed for light and environmental scanning electron microscopic examination. Results: Histological and ultrastructural examination of Group II (Diabetic group) revealed signs of deterioration and degeneration among the filiform and fungiform papillae. After 4 weeks treatment minor to moderate improvement in the architecture of the papillae has been reported in Groups Ill and IV respectively. In comparison to Groups III and IV. oral administration of tamarind seeds extract (Group V) caused significant improvement in the histological structure of the lingual papillae, covering epithelium, their taste buds and the associated gustatory pore. Conclusion: The findings of the present study indicate that aqueous extract of tamarind seeds possessed significant anti-diabetic and anti-inflammatory activity for improvement &diabetes associated oral complications that may prove its beneficial potentiality in treatment of type 2 diabetes

Mona Fathy Abdel Maksoud Eldeeb

01/06/2017

Facial appearance depends on several oral and extraoral factors. The gingiva is an important intraoral tissue which when affected particularly by pigmentation is mainly responsible for the unpleasant appearance. Several causes of gingival pigmentation were previously mentioned in text together with the possible techniques of treatment. In this review, we will focus on this topic with a histological point of view.

Mona Fathy Abdel Maksoud Eldeeb

01/10/2014

Mona Fathy Abdel Maksoud Eldeeb

01/07/2014

Objective: The purpose of this study is to compare the effect of onion and miacalcic on alveolar bone, periodontal ligament (PDL) and cellular cementum in albino rats treated with dexamethasone. Design: Forty male Swiss albino rats ranging from 150-200 gm were selected for the study and randomly divided into four groups: Group I (Control group), Group II (Dexamethasone treated group), Group III (Dexamethasone and Miacalcic treated group) and Group IV (Dexamethasone and Onion treated group). At the end of the experimental period (four weeks), the rats were sacrificed and the mandible specimens were divided into right and left halves. Half of the samples were kept for measuring bone mineral density (BMD). The other halves were prepared for light microscopic examination and immunohistochemical assessment. Results: Histopathological examination of group II revealed severe bone and cementum resorption and disorganization of PDL. Less resorptive activity and more dense and organized PDL could be detected in group III, while group IV showed normal architecture closely resembling that of the control group. The greatest fibronectin (FN) expression and the highest mean of BMD values were demonstrated in the onion treated group (group IV) which was nearly similar to that of the control group. Conclusions: Onion treatment when combined with dexamethasone prevented alveolar bone and cementum resorption to a marked degree, preserved the density of the alveolar bone, and maintained the PDL architecture to a level comparable to the control.

Mona Fathy Abdel Maksoud Eldeeb

Rehab A. AbdelMoneim

01/07/2014

Mona Fathy Abdel Maksoud Eldeeb

Saher Sayed Ali, Ali Abdel-Halim Abdel-Azim, Mohamed Taha Shredah

01/05/2013

Mona Fathy Abdel Maksoud Eldeeb

Samah S. Mehanni, Hanan M. R. Shokier

01/04/2013

Most of the temporomandibular joint disorders (TMJ) are related to malposition and deformity of the articular disc as in disc displacement with or without reduction as well as complete union of the articulating surfaces as in ankyloses. Most of these disorders result in malorientation and degeneration of disc architecture leading to loss of its function. On the other hand, surgical preservation of TMJ discs in case of disc displacement and ankylosis were proposed to decrease friction and decrease the rate of recurrence. Therefore, this study aimed at assessment of histopathological changes of resected discs during management of anterior displacement without reduction (ADDwoR) and ankylosis to appraise its structure accommodation to function after trauma. Histopathological and Immunohistochemical analyses of resected discs collected from ten patients with either ADDwoR or ankylosis were performed at different bands of discs using anti-apoptotic protein, surviving, as degenerative expression. The anterior band of the ankylosed discs showed more degenerative cartilaginous matrix changes than the ADDwoR. Although both cases revealed cartilaginous matrix in the intermediate zone (the highest surviving expression), ankylosis only was invaded by blood vessels. On the other hand, the posterior disc attachment (PDA) appeared more degenerated in ADDwoR rather than in ankylosis. It is concluded that, the degeneration of discs in both ADDwoR and ankylosis are reflected by their surviving expression interfere with disc function. In addition, the detected high vascularity of the ankylosed discs may act as nidus for reankylosis.

Mona Fathy Abdel Maksoud Eldeeb

Mohamed Taha Shredah

01/10/2012

Azathioprine (AZA) is a therapeutic compound widely administered in the clinic for its multiple uses in treatment of autoimmune diseases, post-transplant immunosuppression and cancer. Despite these advantages, its therapeutic potential is limited by occasional adverse effects as myelotoxicity and hepatotoxicity. Aim: Is to study the molecular effect of AZA on mice gingiva. Material and method: Thirty six adult male mice (CD- I ) of average weight 100-150 gm each were used in the present work, they were divided into two main groups. the study group comprises twenty four animals and the control group comprises twelve animals. Animals of the study groupreceived a daily therapeutic dose of AZA dissolved in saline solution containing 5% Tween-80 and injected intraperitoneally at a dose of 3 mg/kg body weight .The study group was subdivided into 2 subgroups (12 animals each) according to the time of euthanasia which is one month group (group 2) and two months group (group 3). Animals of the control group (group 1) were injected intraperitoneally an equivalent volume of normal saline-Tween-80 and subdivided into 2 subgroups (6 animals each), which matched the study group in the time of euthanasia. At the time of euthanasia, the gingiva was dissected from each mouse, washed and kept frozen for molecular study. Results: DNA showed more destruction in group 3 than in group 2 as seen on gel electrophoresis. Conclusion: The metabolic products of AZA incorporate with the DNA causing destruction and damage with severe molecular pathology.